Tuesday, May 24, 2016

Thank you ATS!

Thank you to the American Thoracic Society (ATS) conference organizers for another wonderful event! Our team is heading back to the office and are looking forward to continuing the discussion with all the researchers we met at our booth.

E-cigarette research

We had a wonderful Breakfast Symposium last Monday where guest speaker Dr. Laura Crotty Alexander1, SCIREQ's Vice-President Mr. David Brunet and Senior Scientist Dr. Annette Robichaud discussed what is known about e-cigarettes and what is left to be discovered and how researchers might work together towards standardization of the various parameters used to assess the impact of these devices. Many researchers gathered to share their knowledge and to begin answering some questions. Everyone agrees that more work needs to be done before we can have a clear understanding of the impact of e-cigarettes - an industry that it changing at a fast pace.

Lung volumes

The flexiVent is now capable of providing more measurements of lung volumes. The newest additions include measurements of the total and residual lung volumes (TLC/RV). As with other techniques, this is done using a computer-controlled automated manoeuvre for standardization and control of parameters. The acquisition of these new outcomes does not require the use of a separate device and can easily be preceded by comprehensive respiratory mechanics measurements typically performed with the flexiVent. Lung volume changes are sensitive to physiological or pathophysiological changes.

SCIREQ's Senior Scientist, Dr. Annette Robichaud, and R&B manager, Ms. Liah Fereydoonzad, presented their poster at ATS entitled "Automated full range pressure-volume curves in mice & rats2" which found that the automated method of constructing full range PV curves in mice proved to be equivalent to the classic, manually operated, syringe pump method. The automation of the technique alleviates some of the weaknesses of the classic technique while bringing simplicity and standardization.

To learn more on the flexiVent and the lung volumes technique, visit our website at www.scireq.com/flexiVent/lung_volumes.

1Dr. Laura Crotty Alexander is a researcher and staff physician in the VA San Diego Healthcare System and Assistant Professor at the University of California at San Diego (UCSD). She obtained her doctorate degree of medicine (M.D.) from Duke University and trained in internal medicine at the Massachusetts General Hospital. She then stayed in the Boston area and completed a Pulmonary and Critical Care fellowship training at the Harvard Medical School before moving to UCSD for a post-doctoral fellowship. She obtained a VA career development award via her research in asthma, and accepted a faculty position at UCSD in 2011.

Dr. Crotty Alexander has authored many publications on electronic cigarettes and is a leader in this field of research.

2Automated full range pressure-volume curves in mice & rats - Robichaud A et al. PDF version on SCIREQ website

Thursday, May 19, 2016

inExpose featured in recent Nature publication

Congratulations to Dr. Blackwell's team at Vanderbilt University who recently published in Nature Communications1!

As you may know, chronic obstructive pulmonary disease (COPD) is characterized by obstruction in airflow, chronic inflammation, and destruction of the alveolar tissue. This group from Vanderbilt University hypothesized that secretory immunoglobulin A (SIgA) deficiency contributes to chronic airway inflammation and COPD disease progression.

The SCIREQ inExpose was utilized in multiple ways to gather evidence to support their theory. First, using the inExpose to deliver cigarette smoke, researchers confirmed that (SIgA) knock-out mice spontaneously develop COPD-like symptoms with similar severity to cigarette smoke exposed subjects.

Next, a lysate from a non-typeable Haemophilus influenza (NTHi), which is commonly found in COPD patients, was prepared as an aerosol solution and delivered using the inExpose to both wild-type and knock-out mice. The group then re-introduced SIgA and found an attenuated inflammatory response, suggesting SIgA limits the response to bacterial antigens in the respiratory system.

To learn more and read the full publication, visit Nature Communications' website at www.nature.com/ncomms/2016/160405/ncomms11240/

Whether through smoke or aerosol, nose-only or whole body exposure, the inExpose easily adapts to various toxicology research needs by permitting relevant and reproducible inhalation exposure models. To learn more on this product, please visit our website at www.scireq.com/inexpose.

1Airway bacteria drive a progressive COPD-like phenotype in mice with polymeric immunoglobulin receptor deficiency Richmond BW et al. Nat Commun. 2016 Apr 5;7:11240. doi: 10.1038/ncomms11240.

Tuesday, April 26, 2016

Japanese Respiratory Society's annual meeting with a global focus

We were delighted to see a global presence at the 56th Annual Meeting of the Japanese Respiratory Society (JRS) conference in Kyoto Japan. The conference, themed "Pulmonary medicine 2016, Developing Intellectual and Future Innovations", featured ATS/ERS/JRS/APSR Inter-Assembly Symposiums that aimed to promote basic lung research and strengthen exchanges with academic societies overseas.

Many International speakers were invited as lecturers to highlight important progress being made in the field of respiratory research. Key International symposiums included:

  • Update on lung cancer
  • Inter-assembly symposium on Allergy, Immunology and inflammation
  • Inter-assembly symposium of Respiratory Cell and Molecular Biology
  • Update on COPD pathogenesis and therapy
  • Update on asthma pathogenesis and therapy
  • Update on Pulmonary Fibrosis and therapy

    Mr. Eiji Iwane and Shota Inoue, emka & SCIREQ's representative in Japan, and SCIREQ's Vice President Mr. David Brunet met with researchers and delegates from the various respiratory societies, taking part in this international collaboration first hand.

    We loved Japan and hope all JRS attendees enjoyed its amazing culture, food and views of Sakura cherry blossom trees. We hope to see you in Tokyo next year.

    Related links:

  • Japanese Respiratory Society (JRS)
  • American Thoracic Society (ATS)
  • European Respiratory Society (ERS)
  • Asian Pacific Society of Respirology (APSR)

  • Thursday, March 24, 2016

    Meet with us in San Diego at Experimental Biology!

    Our team of Application Specialist are traveling to sunny San Diego next week to attend the Experimental Biology conference yet again! We will present and demonstrate our solutions for physiology, pharmacology and toxicology research. Take advantage of our presence to discuss your research and how emka & SCIREQ can provide solutions for your studies.

  • Detailed, reproducible and accurate data with the flexiVent, the gold standard for in vivo lung function measurements.
  • Inhalation exposure studies with e-cigarettes, heavy metals, environmental pollutants, occupational hazards and more using the inExpose.
  • tremoFlo for detailed lung function measurements in conscious large animals that go far beyond the guideline.
  • Plethysmographs for control of breathing and other conscious respiratory measurements.

    Click here to schedule a meeting with one of our respiratory specialists during EB 2016.

    Exhibit hours:

    Sunday April 3 - 9am to 4pm
    Monday April 4 - 9am to 4pm
    Tuesday April 5 - 9am to 4pm

    Contact us!

    Phone 1.514.286.1429 | Toll Free 1.877.572.4737
    Email emka_SCIREQ@scireq.com

  • Tuesday, March 8, 2016

    emka & SCIREQ at ToxExpo 2016 in New Orleans!

    We will present and demonstrate our solutions for physiology, pharmacology and toxicology research. Take advantage of our presence to discuss your research and how emka & SCIREQ can provide solutions for your studies.

  • Inhalation exposure studies with e-cigarettes, heavy metals, environmental pollutants, occupational hazards and more using the inExpose
  • tremoFlo for detailed lung function measurements in conscious large animals that go far beyond the guideline.
  • Plethysmographs for conscious toxicology studies compliant with ICH S7A Guidelines & GLP

    As experts in respiratory safety pharmacology and toxicology emka & SCIREQ offer a suite of precise preclinical instruments for ex vivo and in vivo studies. Talk to our experienced team at SOT ToxExpo 2016 in New Orleans, LA at booth 1439, during posters and scientific sessions. Take advantage of our daily live demos to learn more about our products and services which include GLP guideline compliance.

    Booth 1439

    Exhibit hours:
    Monday March 14 9:15am to 4:30pm
    Tuesday March 15 9:15am to 4:30pm
    Wednesday March 16 9:15am to 4:30pm

    Contact us to schedule a meeting at our booth!

    Phone 1.514.286.1429 | Toll Free 1.877.572.4737
    Email emka_SCIREQ@scireq.com

  • Monday, February 22, 2016

    WHAT YOU ALWAYS WANTED TO KNOW ABOUT SPECIFIC AIRWAY RESISTANCE

    Specific airway resistance (sRaw), or its reciprocal specific airway conductance (sGaw), was first introduced over 35 years ago as a way to gather information on airflow resistance in conscious subjects during quiet spontaneous breathing. This parameter is derived from the double-chamber plethysmography (DCP) technique, which separately captures the subject’s nasal and thoracic flow signals over time.

    sRaw and the DCP Technique

    In addition to information on flow, information on the alveolar pressure changes is typically needed to characterise resistance to airflow within the respiratory system. This latter signal is however not easy to record in a conscious subject. In the DCP technique, information on airflow resistance across the entire airway tree is extracted from the comparison of nasal and thoracic flow waveforms. In this approach, while both waveforms are generally similar and can be superimposed under baseline conditions, the presence of an increased resistance, such as during bronchoconstriction, causes the nasal signal to be delayed relative to the thoracic one, thus inducing a measurable time interval that was shown to be proportional to sRaw.

    sRaw and Gas Conditioning

    The point on the waveform at which to characterize the time delay between nasal and thoracic flow signals is important. During inspiration, the air entering the airways gets heated and humidified and, as a result, expands within the lungs. This process, known as gas conditioning, has significant implications in the determination of the time delay mainly because it is particularly important during inspiration, which is also when peak changes in resistance occur. However, gas conditioning is proportional to flow. Therefore, its impact is minimal at the end of inspiration where flow is zero (see red circle in the picture). This specific point on the flow profile can thus be employed for sRaw determination, specifically because of it allows for a separation between the contributing gas conditioning and resistive components. It is worth mentioning that this specific point does not however correspond to the point of peak resistance changes, which would happen earlier during inspiration, and therefore represents a compromise as highlighted by the phenotyping uncertainty principle.

    sRaw and Airway Resistance

    While sRaw captures information on airflow resistance, it is important to realize that it is not a true measurement of airway resistance. Its units are cmH2O.s while typical airway resistance units would be cmH2O.s/mL. sRaw rather denotes work of breathing as it is defined by the product of airway resistance and the lung volume at the end of inspiration, the functional residual capacity or FRC. Since both factors can influence the outcome of sRaw and that there is an inverse relationship between them, best practice would be to complement measurements of sRaw with direct measurements of both upper and lower airway resistance. This would not only provide more insight in interpreting the results but also a refined and comprehensive assessment leading to a deeper understanding. The flexiVent system offers detailed lung function measurements which can prove helpful in identifying the response site within the lower airway segment while also providing direct upper airway resistance measurements. Both airway segments can also be assessed almost simultaneously in the same subject using a specialized configuration of the system.

    Please contact us to find out more on preclinical lung function measurement techniques and their outcomes.

    Read more

    Pennock et al. 1979. A noninvasive technique for measurement of changes in specific airway resistance. J Appl Physiol 46: 399-406.

    Bates JH, Irvin CG. 2003. Measuring lung function in mice: the phenotyping uncertainty principle. J Appl Physiol 94:1297-306.

    West, JB, 2012. Respiratory physiology: the essentials. Lippincott Williams & Wilkins.

    Thursday, February 18, 2016

    Customer service tailored to your research application

    It has been almost two years since emka & SCIREQ joined forces to offer a wider range of scientific instruments for life sciences research. To ensure you receive the best application support, our team is now structured by area of expertise, aligned with your research interests for both non-GLP and GLP applications:

  • SCIREQ: Respiratory research - lung function and inhalation
    www.scireq.com | sales@scireq.com | 1-877-572-4737

  • emka:Telemetry, cardiovascular and neuro studies
    www.emkatech.com | emkatech@emkatech.com | 703-237-9001

    Dedicated emka & SCIREQ representatives are always available to discuss your research and offer the best tools for your application. Please do not hesitate to contact us with your inquiries.