HOLIDAY OFFICE HOURS
Closed from Monday, December 26th to Friday, December 30th.
Closed on Monday, December 26th to Monday, January 2nd only. Open all other days.
Closed from Monday, December 26th to Monday, January 2nd.
Thank you to the American Thoracic Society (ATS) conference organizers for another wonderful event! Our team is heading back to the office and are looking forward to continuing the discussion with all the researchers we met at our booth.
SCIREQ's Senior Scientist, Dr. Annette Robichaud, and R&B manager, Ms. Liah Fereydoonzad, presented their poster at ATS entitled "Automated full range pressure-volume curves in mice & rats2" which found that the automated method of constructing full range PV curves in mice proved to be equivalent to the classic, manually operated, syringe pump method. The automation of the technique alleviates some of the weaknesses of the classic technique while bringing simplicity and standardization.
To learn more on the flexiVent and the lung volumes technique, visit our website at www.scireq.com/flexiVent/lung_volumes.
1Dr. Laura Crotty Alexander is a researcher and staff physician in the VA San Diego Healthcare System and Assistant Professor at the University of California at San Diego (UCSD). She obtained her doctorate degree of medicine (M.D.) from Duke University and trained in internal medicine at the Massachusetts General Hospital. She then stayed in the Boston area and completed a Pulmonary and Critical Care fellowship training at the Harvard Medical School before moving to UCSD for a post-doctoral fellowship. She obtained a VA career development award via her research in asthma, and accepted a faculty position at UCSD in 2011.
Dr. Crotty Alexander has authored many publications on electronic cigarettes and is a leader in this field of research.
2Automated full range pressure-volume curves in mice & rats - Robichaud A et al. PDF version on SCIREQ website
Congratulations to Dr. Blackwell's team at Vanderbilt University who recently published in Nature Communications1!
As you may know, chronic obstructive pulmonary disease (COPD) is characterized by obstruction in airflow, chronic inflammation, and destruction of the alveolar tissue. This group from Vanderbilt University hypothesized that secretory immunoglobulin A (SIgA) deficiency contributes to chronic airway inflammation and COPD disease progression.
The SCIREQ inExpose was utilized in multiple ways to gather evidence to support their theory. First, using the inExpose to deliver cigarette smoke, researchers confirmed that (SIgA) knock-out mice spontaneously develop COPD-like symptoms with similar severity to cigarette smoke exposed subjects.
Next, a lysate from a non-typeable Haemophilus influenza (NTHi), which is commonly found in COPD patients, was prepared as an aerosol solution and delivered using the inExpose to both wild-type and knock-out mice. The group then re-introduced SIgA and found an attenuated inflammatory response, suggesting SIgA limits the response to bacterial antigens in the respiratory system.
To learn more and read the full publication, visit Nature Communications' website at www.nature.com/ncomms/2016/160405/ncomms11240/
Whether through smoke or aerosol, nose-only or whole body exposure, the inExpose easily adapts to various toxicology research needs by permitting relevant and reproducible inhalation exposure models. To learn more on this product, please visit our website at www.scireq.com/inexpose.
1Airway bacteria drive a progressive COPD-like phenotype in mice with polymeric immunoglobulin receptor deficiency Richmond BW et al. Nat Commun. 2016 Apr 5;7:11240. doi: 10.1038/ncomms11240.
We were delighted to see a global presence at the 56th Annual Meeting of the Japanese Respiratory Society (JRS) conference in Kyoto Japan. The conference, themed "Pulmonary medicine 2016, Developing Intellectual and Future Innovations", featured ATS/ERS/JRS/APSR Inter-Assembly Symposiums that aimed to promote basic lung research and strengthen exchanges with academic societies overseas.
Many International speakers were invited as lecturers to highlight important progress being made in the field of respiratory research. Key International symposiums included:
Mr. Eiji Iwane and Shota Inoue, emka & SCIREQ's representative in Japan, and SCIREQ's Vice President Mr. David Brunet met with researchers and delegates from the various respiratory societies, taking part in this international collaboration first hand.
We loved Japan and hope all JRS attendees enjoyed its amazing culture, food and views of Sakura cherry blossom trees. We hope to see you in Tokyo next year.
Our team of Application Specialist are traveling to sunny San Diego next week to attend the Experimental Biology conference yet again! We will present and demonstrate our solutions for physiology, pharmacology and toxicology research. Take advantage of our presence to discuss your research and how emka & SCIREQ can provide solutions for your studies.
Click here to schedule a meeting with one of our respiratory specialists during EB 2016.
Phone 1.514.286.1429 | Toll Free 1.877.572.4737
Email [email protected]
We will present and demonstrate our solutions for physiology, pharmacology and toxicology research. Take advantage of our presence to discuss your research and how emka & SCIREQ can provide solutions for your studies.
As experts in respiratory safety pharmacology and toxicology emka & SCIREQ offer a suite of precise preclinical instruments for ex vivo and in vivo studies. Talk to our experienced team at SOT ToxExpo 2016 in New Orleans, LA at booth 1439, during posters and scientific sessions. Take advantage of our daily live demos to learn more about our products and services which include GLP guideline compliance.
Contact us to schedule a meeting at our booth!
Phone 1.514.286.1429 | Toll Free 1.877.572.4737
Email [email protected]
Specific airway resistance (sRaw), or its reciprocal specific airway conductance (sGaw), was first introduced over 35 years ago as a way to gather information on airflow resistance in conscious subjects during quiet spontaneous breathing. This parameter is derived from the double-chamber plethysmography (DCP) technique, which separately captures the subject’s nasal and thoracic flow signals over time.
In addition to information on flow, information on the alveolar pressure changes is typically needed to characterise resistance to airflow within the respiratory system. This latter signal is however not easy to record in a conscious subject. In the DCP technique, information on airflow resistance across the entire airway tree is extracted from the comparison of nasal and thoracic flow waveforms. In this approach, while both waveforms are generally similar and can be superimposed under baseline conditions, the presence of an increased resistance, such as during bronchoconstriction, causes the nasal signal to be delayed relative to the thoracic one, thus inducing a measurable time interval that was shown to be proportional to sRaw.
The point on the waveform at which to characterize the time delay between nasal and thoracic flow signals is important. During inspiration, the air entering the airways gets heated and humidified and, as a result, expands within the lungs. This process, known as gas conditioning, has significant implications in the determination of the time delay mainly because it is particularly important during inspiration, which is also when peak changes in resistance occur. However, gas conditioning is proportional to flow. Therefore, its impact is minimal at the end of inspiration where flow is zero (see red circle in the picture). This specific point on the flow profile can thus be employed for sRaw determination, specifically because of it allows for a separation between the contributing gas conditioning and resistive components. It is worth mentioning that this specific point does not however correspond to the point of peak resistance changes, which would happen earlier during inspiration, and therefore represents a compromise as highlighted by the phenotyping uncertainty principle.
While sRaw captures information on airflow resistance, it is important to realize that it is not a true measurement of airway resistance. Its units are cmH2O.s while typical airway resistance units would be cmH2O.s/mL. sRaw rather denotes work of breathing as it is defined by the product of airway resistance and the lung volume at the end of inspiration, the functional residual capacity or FRC. Since both factors can influence the outcome of sRaw and that there is an inverse relationship between them, best practice would be to complement measurements of sRaw with direct measurements of both upper and lower airway resistance. This would not only provide more insight in interpreting the results but also a refined and comprehensive assessment leading to a deeper understanding. The flexiVent system offers detailed lung function measurements which can prove helpful in identifying the response site within the lower airway segment while also providing direct upper airway resistance measurements. Both airway segments can also be assessed almost simultaneously in the same subject using a specialized configuration of the system.
Please contact us to find out more on preclinical lung function measurement techniques and their outcomes.
Pennock et al. 1979. A noninvasive technique for measurement of changes in specific airway resistance. J Appl Physiol 46: 399-406.
Bates JH, Irvin CG. 2003. Measuring lung function in mice: the phenotyping uncertainty principle. J Appl Physiol 94:1297-306.
West, JB, 2012. Respiratory physiology: the essentials. Lippincott Williams & Wilkins.
It has been almost two years since emka & SCIREQ joined forces to offer a wider range of scientific instruments for life sciences research. To ensure you receive the best application support, our team is now structured by area of expertise, aligned with your research interests for both non-GLP and GLP applications:
Dedicated emka & SCIREQ representatives are always available to discuss your research and offer the best tools for your application. Please do not hesitate to contact us with your inquiries.
The New Year has just started and already an impressive number of publications featuring the flexiVent are available. Amongst these first 2016 publications, we are proud to highlight one by a Canadian group of researchers whose findings could prove to be useful to outline disease mechanisms or investigate new therapeutic avenues:
BIOSIGNATURE FOR AIRWAY INFLAMMATION IN A HOUSE DUST MITE-CHALLENGED MURINE MODEL OF ALLERGIC ASTHMA. - Hadeesha Piyadasa, Anthony Altieri, Sujata Basu, Jacquie Schwartz, Andrew J. Halayko, Neeloffer Mookherjee. Biology Open 2016 : doi: 10.1242/bio.014464
The study describes a list of biomolecules pertaining to the allergen most frequently associated with human asthma, house-dust mite, in a preclinical model. It also includes relevant physiological endpoints such as airway hyperresponsiveness, a key feature of asthma, which, in this paper, was assessed using a detailed lung function measurement method exclusive to the flexiVent system. This approach is based on a multi-frequency forced oscillation evaluation of the respiratory system which permits the partitioning of the response into airway and tissue mechanics. A refined level of model characterization can thus be obtained for a deeper level of understanding.
Please contact us for more information on how to incorporate detailed lung function measurements into your studies.
We invite you to click here and browse through over 1,500 publications featuring the flexiVent.